Three-Dimensional Structures of Translating Ribosomes by Cryo-EM

Modeling Protein Structure Features from Three Dimensional Cryo-EM Images

Secondary structure of protein, such as α-helix and βsheet, is the general three-dimensional (3D) form of local segments. It can be identified from the 3D electron cryomicroscopy (cryo-EM) density images at medium resolutions (~5-10Å). A detected β-sheet can be represented by either the voxels of β-sheet density or by many piecewise polygons to compose a rough surface. However, none of these is...

Three-Dimensional Structure Determination from Common Lines in Cryo-EM by Eigenvectors and Semidefinite Programming

The cryo-electron microscopy reconstruction problem is to find the three-dimensional (3D) structure of a macromolecule given noisy samples of its two-dimensional projection images at unknown random directions. Present algorithms for finding an initial 3D structure model are based on the "angular reconstitution" method in which a coordinate system is established from three projections, and the o...

Cryo-EM structures of the autoinhibited E. coli ATP synthase in three rotational states

A molecular model that provides a framework for interpreting the wealth of functional information obtained on the E. coli F-ATP synthase has been generated using cryo-electron microscopy. Three different states that relate to rotation of the enzyme were observed, with the central stalk's ε subunit in an extended autoinhibitory conformation in all three states. The Fo motor comprises of seven tr...

Cryo-EM structures of two bovine adenovirus type 3 intermediates.

Adenoviruses (Ads) infect hosts from all vertebrate species and have been investigated as vaccine vectors. We report here near-atomic structures of two bovine Ad type 3 (BAd3) intermediates obtained by cryo-electron microscopy. A comparison between the two intermediate structures reveals that the differences are localized in the fivefold vertex region, while their facet structures are identical...

Dynamic Analysis of Three Dimensional Asymmetric Isolated Structures